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 Physical Biochemistry Unit Major Interests Our goal is to unravel the physical principles that govern interactions at the molecular level of several clinically relevant compounds with model cell systems, with the objectives of developing new drugs and better understand the mechanism of action of already established ones.
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 Miguel Castanho PhD (1993) in Molecular Biophysics at Technical University, Lisbon Post-doctoral research at Univ. Hawaii, USA, and at Rocasolano Institute in Madrid, Spain Full professor at the Faculty of Medicine, University of Lisbon Awarded by the Ministry of Health, Portuguese Chemical Society, Gulbenkian Foundation and Grünenthal Foundation
2009/2013 - LIPIDENGUE: The role of hepatic lipid membranes and lipid droplets in Dengue virus encapsidation. A gateway to new therapies. (PTDC/QUI-BIQ/112929/2009) 2008/2012 - Toward the development of peptide-based hydrogels for tissue regeneration applications with inherent antibacterial activity. (EC, FP7-PEOPLE-IOF-2008-235154) (FCT, PTDC/SAU-BEB/099142/2008) 2008/ - Elucidating the mechanism of action of cyclotides; ultra-stable proteins from plants. European Comission. PIOF-GA-2008-220318 (joint project with the Institute for Molecular Biosciences, The University of Queensland, Australia) 2007/10 - Targeting kyotorphin derivatives to the brain, FCT (PTDC/SAU-FCF/69493/2006) 2007/10 - The role of lipid membranes on cell infection by Dengue virus, FCT (PTDC/QUI/69937/2006) 2008/10 - Unravelling the mechanism of action of membrane-active peptides combining NMR and optical spectroscopies, CRUP-DAAD (joint project Germany-Portugal A-6/08) 2008/12 - Selected peptides as drug candidates directed to pain and neurodegeneration, EC-FP7 Industry/Academia Partnerships (IAPP) (FP7-PEOPLE-2007-3-1-IAPP, Grant no.: 230654) David Craik (Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia) Andrea Thompson da Poian (Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil) Anne Ulrich (Institute of Biological Interfaces, Forschungszentrum Karlsruhe, Karlsruhe, Germany) Eduard Bardají (Laboratori d’Innovació en Processos i Productes de Síntesi Orgànica - LIPPSO, Departament de Química, Universitat de Girona, Girona, Spain) David Andreu - Proteomics and Protein Chemistry Unit - Department of Experimental Health Sciences(Pompeu Fabra University, Barcelona Biomedical Research Park, Barcelona, Spain) 2010 - Physical Biochemistry co-organized XII EPI - Iberian Peptide Meeting, Lisbon, Portugal, February 2010. Chairman - Miguel ARB Castanho. Castanho, MARB (2007) “Ciência: A Bela está adormeSIDA?”, Seminário “Prevenção da infecção pelo VIH/SIDA e sexualidade(s)” (Santarém, Portugal) Cunha S, Gonçalves R, Correia AD. Apresentação de um novo biomonitor para a avaliação em tempo real da qualidade da água do mar, no 5º Encontro de Ciência e Inovação, Universidade do Porto, 15-18 Março, Pavilhão Rosa Mota, Porto Cunha S, Gonçalves R, Correia AD. Apresentação de um novo biomonitor para a avaliação em tempo real da qualidade da água do mar, sob o Novo Painel de Tecnologias Marinhas, Exposição SEA DAY, 16 Novembro, CIIMAR, Porto 2010 5th International Peptide Symposium, Kyoto, Japan, December 2010 - João Freire and Marta Ribeiro were awarded each with a Best Poster award. 2010 I2P - Ideas to Products, Portugal - COTEC, April 2010 - Marta MB Ribeiro, Henri G Franquelim, Isa Serrano and Miguel ARB Castanho won the COTEC National Competition and ranked 2nd in the COTEC European Competition (RWTH Aachen, Germany) with the M-GATE product. 2009 Grunenthal Award in Pain Research (edition 2008), Portugal (atribuído a Marta Ribeiro, Miguel ARB Castanho and Isaura Tavares (Oporto) "Leading Kyotorphin derivatives to the central nervous system: experimental validation of a novel analgesic molecule") 2007 Prémio de Poster no 6th EBSA European Biophysics Congress (atribuído a Sónia Henriques) 2006 Menção Honrosa – Prémio Grünenthal Dor 2006 - Fundação Grünenthal (atribuído a Miguel Castanho) 2005 Prémio Dr. José Luís Champalimaud - Alto Comissariado para a Saúde (atribuído a Ana Salomé Veiga) 2004 Medalha Vicente Seabra - Soc. Portuguesa de Química (atribuída a Miguel Castanho) 2004 Prémio Dr. José Luís Champalimaud - Com. Nac. Luta contra a SIDA (atribuído a Ana Salomé Veiga) 1996 Prémio Gulbenkian de Iniciação à Investigação Científica - Fotoquímica e Fotofísica (atribuído a Miguel Castanho) Franquelim, H.G. et al. Anti-HIV-1 antibodies 2F5 and 4E10 interact differently with lipids to bind their epitopes. AIDS 25, 419-428 (2011). Franquelim HG, Veiga AS, Weissmüller G, Santos NC, Castanho MA. Unravelling the molecular basis of the selectivity of the HIV-1 fusion inhibitor sifuvirtide towards phosphatidylcholine-rich rigid membranes. Biochim Biophys Acta. 2010 Jun;1798(6):1234-43. Epub 2010 Feb 11 Carla S Alves, Manuel N Melo, Henri G Franquelim, Rafael Ferre, Marta Planas, Lidia Feliu, Eduard Bardají, Wioleta Kowalczyk, David Andreu, Nuno C Santos, Miguel X Fernandes, Miguel A R B Castanho. J Biol Chem (2010) vol. 285 (36) pp. 27536-44 Escherichia coli Cell Surface Perturbation and Disruption Induced by Antimicrobial peptides BP100 and pepR Melo MN, Sousa FJ, Carneiro FA, Castanho MA, Valente AP, Almeida FC, Da Poian AT, Mohana-Borges R. (2009). Interaction of the dengue virus fusion peptide with membranes assessed by NMR: The essential role of the envelope protein TRP101 for membrane fusion. J Mol Biol., in press Ana S. Veiga, Leonard K. Pattenden, Jordan M. Fletcher, Miguel A. R. B. Castanho and Marie Isabel Aguilar (2009). Interactions of HIV-1 antibodies 2F5 and 4E10 with a gp41 epitope prebound to host and viral membrane model systems ChemBioChem, 10, 1032–1044 M. N. Melo, R. Ferre and M. Castanho (2009). Antimicrobial peptides: linking partition, activity and high membrane bound concentrations; Nature Rev. Microbiology, in press R. Ferre, M. N. Melo, A. D. Correia, L. Feliu, E. Bardají, M. Planas and M. Castanho (2009). Synergistic effects of the membrane actions of cecropin-melitin antimicrobial hybrid peptide BP100; Biophys. J., in press H. G. Franquelim, L. Loura, N. C. Santos, M. A. R. B. Castanho (2008) J. Am. Chem. Soc., 130, 6215–6223. Sifuvirtide Screens Rigid Membrane Surfaces. Establishment of a Correlation between Efficacy and Membrane Domain Selectivity among HIV Fusion Inhibitor Peptides F. Stauffer, M. N. Melo, F. A. Carneiro, F. J. R. Sousa, M. A. Juliano, L. Juliano, R. Mohana-Borges, A. T. da Poian, M. A. R. B. Castanho (2008) . Interaction between dengue virus fusion peptide and lipid bilayers depends on peptide clustering. Molec. Membrane Biology, 25, 128-138 Henriques, S.T., Melo, M.N., Castanho, M.A.R.B. (2006) Cell-penetrating peptides and antimicrobial peptides: how different are they? Biochemical Journal, 399, 1-7. Henriques, S., Costa, J., Castanho, M.A.R.B. (2005) Translocation of Ð-galactosidase mediated by the cell-penetrating peptide pep-1 into lipid vesicles and human HeLa cells is driven by membrane electrostatic potential, Biochemistry, 44, 10189-10198 Lopes, S., Goormaghtigh, E., Costa-Cabral, B., Castanho, M. (2004) Filipin orientation revealed by linear dichroism. Implication for a model of action. Journal of the American Chemical Society, 126, 5396-5402. Henriques, S., Castanho, M. (2004) Consequences of Nonlytic Membrane Perturbation to the Translocation of the Cell Penetrating Peptide Pep-1 in Lipidic Vesicles. Biochemistry, 43, 9716-9724. Veiga, A.S., Santos, N.C., Loura, L.M.S., Fedorov, A., Castanho, M.A.R.B. (2004) HIV fusion inhibitor peptide T-1249 is able to insert or adsorb to lipidic bilayers. Putative correlation with improved effi ciency, Journal of the American Chemical Society. 126, 14758-14763. |
